当临床遭遇低肿瘤含量样本,MSI检测如何避免"失准"?
活检样本量不足、穿刺标本肿瘤细胞占比低……这些临床常见困境,常导致微卫星不稳定性(MSI)检测结果失准,使患者错失免疫治疗良机。桐树基因MSI NGS 2249 Panel给出破局答案!基于2249个微卫星位点的定制化NGS Panel,以95.7%准确率、97.2%超敏检出率,重新定义低肿瘤含量样本MSI检测标准!
三大硬核优势,确立行业新标杆!
优势一:超高灵敏度碾压国际主流方案
研究显示与金标准方法(PCR)相比,桐树基因定制MSI NGS 2249 Panel(覆盖2249个微卫星位点),检测灵敏度(97.2%)显著优于FDA批准的MSI NGS检测panel,F1CDx(94.4%)、MSK-IMPACT(95.8%)。
优势二:位点数量决定检测精度
超千位点覆盖可捕获罕见微卫星偏移,位点数量决定精度,位点越多,准确性越强;2249个位点Panel验证“位点数量提升检测精度”的核心优势。避免因位点不足导致的假阴性。
优势三:低肿瘤含量样本的“救星”, MSI NGS 2249 Panel检出率翻倍
在肿瘤细胞含量≤20%的样本中,NGS检出率高达66.7%(PCR仅33.3%),完美解决小样本漏检难题!穿刺活检、液基细胞学等小样本不再因“量少”漏检
Comparison of the MSI-H–positive rate between NGS panel and PCR-based testing. (F) Validation of IHC-MMR for NGS-MSI and PCR-MSI discordant samples. 1 indicates samples in which either the NGS panel or PCR results are not consistent with the MMR-IHC findings. Consistency of MSI status and MMR-IHC results in six inconsistent samples with tumor content <20% (G) and the consistency in four inconsistent samples with tumor content more than 20% (H)